This decrease in TFIIA expression during infection and its dispensability for activation of late but not early genes suggest one of possibly many mechanisms for the transition from viral early to late gene expression. The clinical evolution of our three cases and scientific data in the literature indicate that exclusive folliculosebaceous involvement by herpes, in the setting of a non-vesicular eruption, represents early herpes zoster. (v) Early after HCMV superinfection, 0.3% of cells in the quiescent cultures could be induced to yield infectious HSV-2. Consistent with the involvement of certain cell-cycle-related cellular activities in HSV infection, we have recently shown that cyclin-dependent kinases (cdks) are required for HSV replication, at least in cycling cells (54). In the experiments presented here, we tested this hypothesis by measuring the efficiency of (i) viral replication; (ii) expression of selected immediate-early (IE) (ICP0 and ICP4), early (E) (ICP8 and TK), and late (L) (gC) genes; and (iii) viral DNA synthesis in infected cultures to which Rosco was added after IE or IE and E proteins had already been synthesized. UL41 protein is capable of interacting with a transactivator of an alpha-gene, the alpha-transinducing factor (alpha-TIF). Of 30 baseline HSV-seropositive subjects, 8 developed ≥1 episode of herpes labialis; 1 subject had a primary HSV infection; and 1 subject without baseline serology information had a new diagnosis of genital HSV.
Copyright © 1979 by The American Association of Immunologists, Inc. The transcription rates of the early genes were dramatically reduced in the absence of ICP0. Restricted IE transcription and the absence of viral DNA synthesis favors LAT formation and persistence of the silenced genome. Furthermore, we demonstrate that herpes and influenza A virus infections are enhanced when host circadian rhythms are abolished by disrupting the key clock gene transcription factor Bmal1. The data in this study indicate that the accumulation of viral early (β) and leaky-late (γ1) proteins correlates with the prevention activity. The PKR mutant cell line displayed reduced constitutive and HSV-inducible RANTES expression compared to the control cell line. The herpes simplex virus 1 (HSV-1) immediate early protein ICP22 is recognized primarily as a regulator of viral gene expression.
Absorbed: Journals that are combined with another title. RT-PCR analysis showed that the expression of Egr-1 mRNA in infected SK-N-SH cells gradually increased from 2 through 48 hours p.i. Full text Full text is available as a scanned copy of the original print version. When synthesized in the absence of ICP27, a mutant ICP4 protein was impaired in its ability to repress transcription from the L/ST promoter in the context of viral infection and in vitro. Nuclear runoff transcription analysis demonstrated that the presence of ICP0 resulted in an increase in the transcription rates of the analyzed genes. ICP0 appears to be a multifunctional regulator of gene expression that directly and indirectly interacts with numerous viral and cellular proteins. We conclude that homologous recombination is independent of viral gene functions and that it is likely to be carried out by cellular proteins.
The loss of light sensitivity coincides with the development of resistance to antibodies. 6:2371-2381, 1986). Get a printable copy (PDF file) of the complete article (519K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. The plasmid containing the sequences encoding ICP0 was a more potent transactivator than the plasmid containing the sequences for ICP4. Links to PubMed are also available for Selected References. These observations indicate that both ICP4 and ICP27 can affect the intracellular localization of ICP0.
The plasmid containing the sequences encoding ICP0 was a more potent transactivator than the plasmid containing the sequences for ICP4. However, as a result of varying the amount of de novo protein synthesis after infection, at least three patterns of maximal expression of the IE genes were revealed. The response of three core (truncated) promoters from the herpes simplex virus type 1 IE-4, IE-0, and IE-27 genes to a battery of virus-encoded trans-acting proteins was examined in a short-term transient expression assay system. The basal level of expression from these cassettes differed significantly depending on the extent of 5′-flanking sequence and the cell line that served as host. This article has been cited by other articles in PMC. This article has been cited by other articles in PMC. This article has been cited by other articles in PMC.
This article has been cited by other articles in PMC. Unlabelled: In order to investigate the novel function(s) of the herpes simplex virus 1 (HSV-1) immediate early protein ICP22, we screened for ICP22-binding proteins in HSV-1-infected cells. The interaction of human immunodeficiency virus (HIV) and herpes simplex virus (HSV) was investigated in an acute whole-virus coinfection system. Keratinocytes of the skin or mucosa are the primary entry portals for herpes simplex virus type 1 (HSV-1) in vivo.