Of the remaining 356 isolates, which were untyped by using neutralization test, 348 isolates were analyzed further by RT-PCR targeting the VP1 gene. Classical typing methods will therefore no longer be possible in most instances. Clinical, laboratory and radiologic data were reviewed from their hospital and army medical corps files. Serology – primary infection may be diagnosed by rising antibody titre or the detection of IgM. By using the silica-based RNA extraction method in two out of five samples, enterovirus RNA was detected, whereas four out of five samples were positive following RNA isolation with magnetic silica beads. Environmental surveillance has been conducted in Toyama Prefecture, Japan, four times since 1979 in order to study enteric virus water pollution (18-20). Analysis of the complete VP1 sequence (876 nucleotides) of 136 E30 strains isolated in geographically dispersed regions of the United States and nine other countries between 1956 and 1998 indicated that the currently circulating E30 strains are genetically distinct from those isolated 30 to 40 years ago.
Archives of Virology, Vol. Sera were obtained from participants at 0, 6, 12, 24, and 36 months of age for measuring EV71 neutralizing antibody titers. The NucliSens Basic kit and the Argene Biosoft RT-PCR had comparable sensitivities for detection of enterovirus RNA, and both molecular methods were more sensitive than culture, which detected only 60.5% of positive samples. The most frequent type isolated from the 2011 epidemic was coxsackievirus A type 6 (CVA6), which had ever been one of the main causative agents of herpangina. PV isolates were forwarded to the Regional Reference Polio Laboratory. All of these contribute to more efficient and less technically demanding viral detection in cell culture. Most of E71 strains (16 out of 23) were isolated from the autopsy materials (brains and spinal cords) from 9 children dying of poliomyelitis, meningoencephalitis, and encephalitis.
A cytopathic effect compatible with the presence of an enterovirus after 3 days of incubation occurred in 25 of the 74 (34%) specimens that eventually became positive. & Pallansch, M. Results. Growth characteristics of enteroviruses from 421 specimens were studied. A total of 47 specimens (31 CSF, 12 rectal swabs, 4 throat swabs) from 30 patients with aseptic meningitis were available for the study. A total of 47 specimens (31 CSF, 12 rectal swabs, 4 throat swabs) from 30 patients with aseptic meningitis were available for the study. Also, the probability of isolation of enteroviruses from sewage in Caco-2 cells was 20 times higher that in RD cells.
In four patients with meningitis but without enterovirus detection in CSF, RT-PCR and virus isolation from stools were positive. Altogether enteroviruses were isolated from CSF and/or faecal samples in 29 patients. Differences in pathogenesis for the enteroviruses mainly result from differences in tissue tropism and cytolytic capacity of the virus. Compared to the accepted relative incidence of viral etiologies, aseptic meningitis due to HSV-2 infection was high, and in adult female patients with symptoms of aseptic meningitis, HSV-2 was the virus most commonly detected in the CSF. This publication is from a journal that may support self archiving. The prognoses of meningitis due to enteroviruses are basically good. Objective: To develop on the LightCycler (LC) instrument a real-time RT-PCR assay based on TaqMan technology for the detection of enteroviruses (EV) in cerebrospinal fluid (CSF) specimens.
The highest isolation rate (87.5%) was observed in primary and tertiary monkey kidney cells; on monkey kidney cell lines, human diploid fibroblasts or human heteroploid cells the isolation rate varied between 64% and 71.4%. Altogether enteroviruses were isolated from CSF and/or faecal samples in 29 patients. 208 CSF samples were tested by PCR. Molecular characterization of echovirus 13 samples was achieved by sequence and phylogenetic analyses on partial VP1 sequences from 20 Korean isolates and 10 foreign isolates listed in Genbank. 8(1):E10. Cerebrospinal fluid was examined by polymerase chain reaction for enteroviruses in 30 patients and for herpes simplex virus 1 and 2 in 29 patients. The performance parameters of the GXEA were determined at three sites with 102 cerebrospinal fluid (CSF) samples obtained from patients with suspected meningitis.
Analysis of the complete VP1 sequence (876 nucleotides) of 136 E30 strains isolated in geographically dispersed regions of the United States and nine other countries between 1956 and 1998 indicated that the currently circulating E30 strains are genetically distinct from those isolated 30 to 40 years ago. The serological diagnosis is mostly made with the complement binding reaction (CBR). Connect to ProQuest Once connected, you can view documents in full as well as cite, email or print them. This report is mainly concerned with the isolation of enteroviruses and serological turnout in pediatric patients who visited local practitioners’ officies or general hospitals in Matsue city between May 1969 and March 1971 and were diagnosed or suspected as acute enterovirus infections. Four structural proteins, VP1 to VP4, are assembled to form the virion capsid of icosahedral symmetry.