Furthermore, several studies provide more evidence that enteroviruses are also the causative or contributory agents to chronic diseases including insulin-dependent diabetes mellitus and dilated cadiomyopathy [Hovi et al., 1996; Lönnrot et al., 2000; Zhang et al., 2004]. CPE in 2 to 7 days, positive identification by IF, typed by neutralization IF of NPA specimens Serology – CFT is test of choice, however young children often do not respond with CF antibody after infection. Unfortunately, not all enterovirus types are detected in BGM cells, especially the pathogenic coxsackievirus types A (26), which are difficult to detect; only coxsackievirus A7, A9, and A16 can infect and multiply in BGM cells, whereas other coxsackie A viruses (A1 through A6, A8, A10 through A15, A17 through A22, and A24) are not detected (8). Circles (○) indicate the locations of hospitals where aseptic meningitis patients were admitted. Epidemiologic surveillance for nonpolio EV.National EV surveillance was initiated in the United States in 1961, through cooperation between the Centers for Disease Control and Prevention (CDC) and the state and territorial public health laboratories. Rasmussen, T. The funding organization had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; and preparation, review, or approval of the manuscript.
We and others have recently reported on nucleic acid sequence-based amplification (NASBA) using the NucliSens Basic kit for the diagnosis of EVs (3, 9; F. 59 of this issue). Similarly, the three CVA2 isolates had 8.4–19.5% nucleotide divergence with each other. Although cell cultures can be purchased or prepared in a variety of containers, the 16- by 125-mm glass or plastic round-bottom screw-cap tube is standard, with the cell monolayer adhering from the midpoint to the bottom of one side of the tube. The nasopharyngeal swab specimens were transported in virus transport medium containing Eagle minimum essential medium (MEM) in Hanks balanced salt solution (BSS) with antibiotics (penicillin, 20,000 U/ml; streptomycin 20,000 μl/ml). Norder, H. Finally, a positive CSF viral culture confirms the diagnosis of viral meningitis.
3. [Full Text]. However, there encountered an another fact to be borne in mind that even in seasons other than above mentioned period, actually through the year round, the isolations of vaccine-originated polioviruses had been seen from the feces of the patients who were clinically diagnosed or suspected as aseptic meningitis, febrile disease or exanthematous disease. We systematically analyzed it for epidemiological information as well as trends in laboratory diagnostic techniques. 2). Laboratory tests were negative for alphavirus and CHP virus, and the etiologic agent in a large number of cases was unidentified. To get a comprehensive picture of the impact of RT-PCR in the diagnosis of HEV and HRV infections, we evaluated findings for clinical samples analyzed by virus isolation and RT-PCR during a 5-year period (1996–2000).
Currently genogroups B and C are co-circulating worldwide. The cell-culture is examined periodically for the presence of viral-induced changes such as cytopathic effect (CPE) or the ability to haemadsorb. Morphologically all enteroviruses are alike. Environmental CVB5 and CVB2 strains from this study showed high sequence identity with GenBank global strains. Furthermore, the possibility that JCV, which can cause demyelination in the brain, may also replicate in the brains of multiple sclerosis (MS) patients has not yet been completely excluded. Severe complications include brainstem encephalitis, aseptic meningitis, severe pulmonary oedema and cardio-respiratory collapse 6,7. Copyright © 2014 The British Infection Association.
In a phylogenetic tree, they were directly related to a control virus obtained from a patient hospitalized in 2000 during an outbreak of enterovirus meningitis. Definitive diagnosis of herpes simplex virus encephalitis must be based on virus isolation from brain biopsy material. In the present investigation, we evaluated a rapid method for serotype identification of enteroviruses by indirect immunofluorescence assay (IFA) using commercially available monoclonal antibodies for polioviruses, coxsackieviruses type B, and six serotypes of commonly circulating echoviruses. The highest isolation rate (87.5%) was observed in primary and tertiary monkey kidney cells; on monkey kidney cell lines, human diploid fibroblasts or human heteroploid cells the isolation rate varied between 64% and 71.4%. Development of a single inexpensive enterovirus-specific antigen is thus desirable. 208 CSF samples were tested by PCR. Moreover, the data are used for interpreting trends in enteroviral diseases, such as aseptic meningitis, by associating them with circulating serotypes and can be helpful for studying the association of enteroviruses with clinical manifestations.
Rapid serotype identification of enteroviruses is important in differentiating nonpoliovirus enterovirus pathogens from vaccine strain polioviruses that can be shed for some time after vaccination. Intertypic recombination in the 3D region was detected in a second PV2 (Sabin 2/Sabin 1) and a PV3 (Sabin 3/Sabin 2). In four children, the only blood isolate was from mononuclear leukocytes, and in two, serum was the only positive blood preparation. Of a total of 1663 samples, 131 viral strains were isolated, and 120 of them were identified as enteroviruses, and 11 as adenoviruses.