In the present study, we demonstrate that, although BHV-1 virions devoid of glycoprotein D (BHV-1 gD-/-) still bind to BL-3 cells, they are no longer able to induce apoptosis. Fifteen clinical samples from asymptomatic animals were included as control group. Maximum levels of expression for all proteins were obtained 48 to 72 h post infection of SF-9 cells by recombinant viruses. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. Further characterization of its virulence and the immune responses elicited by it was conducted in cattle. Previously, we have demonstrated that B virus gD polyclonal antibodies were unable to neutralize B virus infectivity on epithelial cell lines, suggesting gD is not required for B virus entry into these cells. The amino-terminal region of glycoprotein C (gC), which is markedly different in each of the viruses, is involved in virus binding to cellular receptors and in interactions with the immune system.
Once connected, you can view documents in full as well as cite, email or print them. In order to locate the functional domain for heparin binding, we expressed the extracellular portion of gB (gBt) and the large subunit of gB (gBb) in Madin Darby bovine kidney (MDBK) cells under the control of the bovine heat shock protein 70A gene promoter. The MAbs were characterized by their reactivity with the gE protein or the gE/gI complex and by competition experiments. After challenge, viral replication in the nasal passages was significantly reduced in animals vaccinated with gIV (10,000-fold) or BHV-1-infected cell lysate (450,000-fold) but just slightly reduced in animals immunized with gI (500-fold) or gIII (25-fold). In this study we investigated the potential of interleukin 12 (IL-12) to further enhance the CTL response. The MAbs were characterized by their reactivity with the gE protein or the gE/gI complex and by competition experiments. Arch.
Results indicate that the gI and gIV glycoproteins were expressed as beta proteins, whereas the gIII glycoprotein was expressed strictly as a gamma protein. This processing step was significantly less efficient in insect cells than the analogous step in mammalian cells, even though the cleavage sites of authentic and recombinant gl were shown to be identical. We hypothesized that an alternative vaccine design strategy that utilized the DNA vaccine pMASIA-tgD as a complex with BNBD3 might improve humoral responses while maintaining robust Th1-type cell-mediated responses. In the presence of monensin, reduced amounts of infectious virus particles were produced, which was mainly due to inhibition of virus release, rather than virus production. We developed a mouse model to assess the immune response elicited by immunization with either a recombinant truncated (tgD) or the authentic full-length (gD) form of BHV-1 gD in VSA3, a novel water-in-oil adjuvant. On the other hand, the excretion of the gE-positive conventional vaccine strain was not reduced and even seemed to be prolonged in the presence of maternal antibodies. The recombinant gBt and gBb were both efficiently secreted from the transfected cells.
Incorporation of the drug did not result in the termination of replicating BHV-1 DNA molecules since radioactively labeled DNA synthesized in drug-treated and untreated cells sedimented at similar rates in alkaline sucrose gradients. Mar6 was completely resistant to neutralization by monoclonal antibody 130-6 in the presence and absence of complement, but was neutralized by polyvalent immune sera. All samples were tested negative for enterovirus, Herpes Simplex virus and West Nile virus. The BoHV-1 isolates were further confirmed by polymerase chain reaction (PCR) using primers specific for glycoprotein B (gB) genomic region. In addition, aspects discussed include a comparison with two other alphaherpesviruses, namely herpes simplex virus and pseudorabies virus. UMH, Université de Mons-Hainaut – Department of Biological Chemistry,Faculty of Science, BEL. Berlin Institute of Technology – Bundcsforschungsanstalt furViruskrankheiten der Tiere, DEU.
By continuing to browse this site you agree to us using cookies as described in About Cookies. No antibody response could be detected in passively immunised calves, whatever the dose used, and they all became BHV-1 seronegative and remained so after dexamethasone treatment (PDT). The protein expressed was identical in molecular mass and antigenic reactivity to the native gIV protein but was localized in the cytoplasm. Wheat germ lectin, Limulus polyphemus lectin and concanavalin A, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. Abstract : Bovine herpesvirus 1 (BoHV1) infects mainly cattle and buffalo causing infectious bovine rhinotracheitis (IBR), infectious pustular vulvovaginitis (IPV) and infectious balanopostitis. Each glycoprotein, whether purified from virus-infected cells or from virus, retained antigenic activity and induced high titers of monospecific antibodies in rabbits. The relative amounts of gI or gIII expressed from the two vectors were similar.
Serum and milk samples were collected at the same time from cattle in BHV1-free herds, cattle in unvaccinated herds, and cattle in herds that were vaccinated twice with a BHV1 marker vaccine.