The reduction in virus growth may be attributable to the loss of functional interaction between gB and the other envelope proteins involved in virus entry, including gD and gH/gL. RNase protection analysis of RNA isolated from the BAL fluid of RacL11-infected mice on day 3 postinfection revealed much higher levels of RNA specific for macrophage inflammatory protein 1alpha (MIP-1alpha), MIP-1beta, and MIP-2 than were observed for KyA-infected mice. Representative Publications: Ahn, B.C., J. EHV-1 lacking UL4P expression was infectious and displayed cell tropism and pathogenic properties in the mouse model similar to those of parental and revertant viruses. In a previous study, an EHV-1 recombinant KyA virus (KgI/gE/75) was generated in which the sequences encoding glycoprotein I (gI) and glycoprotein E (gE) were repaired [Frampton et al. As demonstrated by yeast two-hybrid assays, pORF30 and pORF18 could physically interact, most likely with a 1:1 stoichiometry. Since the functions of the EHV-1 ORF34 and ORF59 protein (pORF34 and pORF59) products are unknown, the overall targets of this thesis are to (i) identify and characterize the EHV-1 pORF34 and pORF59 products and (ii) examine whether they have a role in pathogenesis of EHV-1.

The acute infection was confined to the respiratory tract and blood. Immunohistochemical examination demonstrated that EHV-1 strains A4/72 and A9/92 replicated in neurons of the olfactory bulb, the cortex and the hippocampus. We are also working on the development of procedures that will enable the use of EHV-1 as a vector for immune- and/or gene therapy. Serological results for paired sera from 17 horses suggested, that the initial abortion on 8 February was the index case, and probably caused the other six abortions. Within 24 hr after re-infection, lymphocytes accumulated around the blood vessels, outnumbering monocytes more than twofold, without neutrophils appearing. In a second infection given to four foals, 61 days after the primary inoculation, no clinical signs were observed, haematological changes were minimal and viraemia was absent. Sporadic coughing was also heard.

As demonstrated by yeast two-hybrid assays, pORF30 and pORF18 could physically interact, most likely with a 1:1 stoichiometry. Both humoral and cell-mediated responses to the infection were detected and monitored. These data indicate that gI and/or gI and gE contribute to the ability of EHV-1 to spread directly from cell-to-cell. Vasculitis, the predominant type of lesion in natural infection, and endothelial cell positivity for EHV-1 were detectable only in the lung. Among the areas to benefit will be the search for improved vaccines to protect the breeding industry from equine herpes virus, which can cause abortion, neonatal death and neurological disease, while two studies will focus on the repair of tendon injuries. Cytology of pulmonary washings has been described in one foal with severe pulmonary disease characterized by dyspnea, tachypnea, hypoxemia, and hypercapnia. Sexual health information on genital herpes, an infection caused by either the Type 1 (HSV-1) or Type 2 (HSV-2) herpes simplex virus.

The deletion of IR4 proved to be conditionally lethal as the IR4-null virus replicated in equine non-transformed fibroblast-like cells, but unlike the parent virus, failed to replicate in immortalized cells of murine, rabbit, equine, simian, or human origin. It was concluded that diseases caused by EHV-1 and EHV-4 may be more effectively controlled with a bivalent vaccine containing both EHV-1 and EHV-4 than with the presently used monovalent vaccines based on EHV-1 alone. As a measure of establishment of latency, quantitative DNA PCR showed that the amount of viral DNA within trigeminal ganglia latently infected with UL41NHB was reduced by approximately 30-fold compared with that in KOS-infected ganglia and by 50-fold compared with that in UL41NHB-R-infected ganglia. Overall, the data indicate that immunization with infectious KyA or rgD induces significant levels of cytokines, virus-specific ASC in the lungs and spleen, and long-term virus specific B-cell responses. 2005. It can be also demonstrated within endothelia, in vascular, myometrial, and cardiac myocytes, macrophages, dendritelike cells of lymphoid organs, and chorionic mesenchymal stromal cells. Moreover the isolation of SI vadants among asymptomatic homosexual men is an eady predictor for progression to AIDS independently of CD4 counts.

The Bgl I Pvu II and Hind III DNA fingerprints of the 3 myeloencephalogenic strains appear distinguishable from non-myeloencephalogenic strains. This means that they will not show clinical signs and that they do not normally shed the virus, however, in periods of high stress viral shedding may reoccur (5). Mice infected with KyA lost a negligible amount of body weight (0.18% total weight loss) on day 1 postinfection and regained weight thereafter, whereas mice infected with KyARgp2F or RacL11 steadily lost weight beginning on day 1 and experienced a 20 and 18% loss in body weight, respectively, by day 3. EHV-1 entered the brain through the olfactory neuroepithelium and along the olfactory nerves, and spread transsynaptically in rostro-caudal direction, using olfactory and limbic neuronal networks. Objectives: To assess the utility of placental examination in equine herpesvirus-1 (EHV-1) and EHV-4 abortion diagnosis.