Several herpesvirus glycoproteins are present in the virion envelope and play a role in virus morphogenesis, cell attachment and tropism. We have identified a synthetic peptide derived from the secreted portion of HSV type 2 glycoprotein G, denoted gG-2p20, which has proinflammatory properties in vitro. The gG-2p20 peptide, corresponding to aa 190-205 of glycoprotein G-2, was a chemoattractant for both monocytes and neutrophils in a dose-dependent fashion, and also induced the release of reactive oxygen from these cells. In two patients with HSV-2, the WB result was HSV-1 positive only. The OD450 values obtained are means from duplicate wells. The function of the virion membrane proteins also implies complex interactions. Previous to our study of the sgG-2-derived peptide, indicating proinflammatory activity, the secreted protein had not been ascribed a specific function.
Check this page to see if it is available for download. I was diagnosed at the beginning of march, after exposure two week before. 2014; 6(11):4358-4372. In addition, a type-specific immunoglobulin G (IgG) antibody response was detected in sera from HSV-infected patients by using sgG-2 as the antigen (18). 4: 110. A number of workers have described the construction of libraries on the surface of M13 bacteriophages and the application of these libraries to a broad range of pathogens (4, 7, 15). In agreement with the expected prevalence of HSV-2 infection, 27.6% of 134 serum samples from random normal individuals had antibodies reactive with recombinant gG.
However, HSV-1 and HSV-2 infections elicit extensive serological cross-reactivity due to their high degree of genetic similarity, which makes it difficult to use serum antibody tests for HSV classification. In contrast, 13 alpha C6 detected the 72K cleavage product and the uncleaved precursor but not the 31K cleavage component. A reliable type-specific diagnosis may be of importance for several reasons: (i) for optimal dosage of antiviral treatment since sensitivities to antivirals differ between the two viral subtypes (10), (ii) for counseling of couples where one of the partners has genital herpes, (iii) to provide means for HSV seroepidemiological studies to be based on seroassays of high specificity and sensitivity (13), and (iv) to evaluate efficacy during trials of HSV prophylactic agents, including vaccines, by determining frequencies of type-specific seroconversions. Accepted 11 May 2003. In conclusion, we have shown that immunization with the type-specific mgG-2 protein in combination with CpG could elicit protective immunity against an otherwise lethal vaginal HSV-2 challenge. The affinity constants were within the range of 10(7)-10(8)M(-1) proving their high-affinity. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards.
Most HSV infections are transmitted in the absence of lesions (Bryson et al., 1993). The study indicates that gG321–580His has a high diagnostic potential for HSV-2 virus serodiagnosis in humans. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. You don’t know if you fall in this category. In this work, we addressed whether SgG2 could contribute to HSV-2-induced pain. Sensitivity for HSV-1 was 95%; specificity was 96%. The gG-2p20 peptide, corresponding to aa 190–205 of glycoprotein G-2, was a chemoattractant for both monocytes and neutrophils in a dose-dependent fashion, and also induced the release of reactive oxygen from these cells.
HindIII l was found to comprise 9629 base pairs. Three gG-related recombinant baculovirus expressed peptides of 37, 42, and 44 kDa were detected by Western blotting using monoclonal antibody to gG. These cells are highly polarized, and the protein composition of their apical and basolateral membranes are very different, so that different viral entry pathways have evolved for each surface. PNAS is the world’s most-cited multidisciplinary scientific serial. Glycoprotein G (gG-2) of herpes simplex virus type 2 (HSV-2) is cleaved to a secreted amino-terminal portion and to a cell-associated, heavily O-glycosylated carboxy-terminal portion that constitutes the mature gG-2 (mgG-2). Viruses have been extensively used for transferring genes into cells both in vitro and in vivo and remain the most efficient tools available for gene therapy applications. This is a short preview of the document.
Please check the format of the address you have entered. A Herpes Simplex Virus-1 (HSV-1) ELISA IgG kit for qualitative detection of type-specific IgG antibodies to HSV-1 in human serum using purified recombinant HSV-1 glycoprotein-G antigen (gG1). Genital herpes is a painful disease frequently caused by the neurotropic pathogen herpes simplex virus type 2 (HSV-2). Herpes simplex virus type 2 glycoprotein gG-2 undergoes a cleavage event during its synthesis and processing. Variants of herpes simplex virus type 2 (HSV-2) generated by virus passage in GMK-AH1 cells in the presence of the sulfated oligosaccharide PI-88 were analyzed. We have identified a synthetic peptide derived from the secreted portion of HSV type 2 glycoprotein G, denoted gG-2p20, which has proinflammatory properties in vitro.