Nigrelli RF, 1952. Regardless of the show style, fish returning from shows should be quarantined for a minimum of 30 days and held at 75°F prior to being placed back into the general population. 5). Supporting the latter hypothesis, the expression level of IL-6 at day 8 post-inoculation was significantly higher in the FL BAC revertant group as compared to the FL BAC revertant ORF134 Rev group. Set II contains 100 junctions representing the 92 donor and 52 acceptor sites marked in . To confirm that selection was specific for IgM+ B cells, presorted WBC, IgM selected, and nonselected cells were costained by antibodies against IgM and transcription factor Pax5, an additional marker specific for B cells (36), and analyzed by FACS. 2004, Yoshino et al.
borreli lysate (concentration equivalent to 0.5 parasite/cell) for 6 d at 27°C in the presence of 5% CO2. However, this seems improbable in this case, since herpes and pox viruses propagate in different cell compartments. In nearly all locations, most transcription corresponded to expression of predicted protein-coding regions. KHV inoculation of fish.Specific-pathogen-free common carp (C. 0003a) from the same 13 individuals, indicating that each of the CyHV-3 genomes detected existed in the form of linear DNA. LAMP products could be detected by the naked eye with the aid of SYBR Green I stain, which could facilitate field implementation of the LAMP assay. Previously we sequenced CyHV-3 fragments obtained from Yura River, Japan, and the sequence also matched that of the Japanese strain (11).
As no replication of the CyHV-3 genome takes place, nor does its segregation to host daughter cells (Ilouze et al., 2012b), the concentration of the viral genome in the host tissues would be too low to be detected during the latent phase. AngHV-1 ORF49, presumably encoding a type 3 membrane protein, was detected in very low abundance in both complete virion analyses. The positive samples tested by nested PCR also gave positive reactions with LAMP, and no sample that was negative by nested PCR tested positive with the LAMP assay, which proved the applicability of the assay in CyHV-2 disease diagnosis. When fish samples were tested, the LAMP assay results correlated strongly with nested PCR results for CyHV-2 detection (data not shown). Probes were also designed to target an exogenous spike-in control (a synthetic RNA oligonucleotide based on Arabidopsis thaliana miRNA ath-miR15.6g, miRBase accession MIMAT0001012) and several endogenous positive controls (host miRNA sequences ccr-miR-21, ccr-miR-22a, ccr-let-7a and ccr-et-7b; miRBase accessions MIMAT0026267, MIMAT0026275, MIMAT0026189 and MIMAT0026190 respectively). Counting the terminal repeat once, KHV has an ORF density of 0.57 per kbp, somewhat lower than IcHV-1 (0.68 per kbp), even though the great majority of the genome is predicted to be protein coding. 32(5): 164-173 .
The plates were washed a final time and kept in the dark at 4 °C prior to reading the fluorescence in a Synergy HT spectrophotometer (Fisher Scientific, Leicestershire, UK). Spring viremia of carp (SVC). Serum testosterone was measured by competitive ELISA using a Testosterone EIA Kit (Cayman Chemical Company, Ann Arbor, MI, USA) according to the manufacturer’s instructions. The DNA concentration was adjusted to 0.1 μg/μl before use in PCR. The sensitivity of each LAMP and PCR were compared using 10-fold serial dilutions of the KHV suspension (the supernatant of the KF-1 culture). Staining using the Halls bile method was negative. 1997a).
Furthermore, transmission electron microscopy studies performed at WAVDL have revealed that samples from koi positive for CEV by PCR also yield poxvirus-like virions (Fig. Microbial Ecology 51(3):277-284 Link 著書 内井喜美子・川端善一郎 (2016) コイヘルペスウイルス. The carp pox virus, in common with other viruses, is far smaller than a bacterium and consists of a piece of genetic material such as RNA or DNA. Among the Herpesvirales order, vIL-10s have been described in members of the Herpesviridae (e.g. Six ORFs encode proteins with closest relatives in virus families such as Poxviridae and Iridoviridae (9). (EN) Herpesviridae, su MicrobeWiki. View Full Text PDF Listings View primary source full text article PDFs.
Four physical treatments were applied on a defined area of the carp epidermis (disc shape, diameter of 15 mm): rubbing with a soft tissue paper (TORK premium, Goteborg, Sweden), rubbing with a cotton swab (Swube Applicator, Becton Dickinson Microbiology system, Maryland, USA), brushing with a rotary electric tooth brush (Philips Sensiflex HX 1513, Anderlecht, Belgium) for 2 s or rubbing with sandpaper (average particle diameter of 265 μm, Medium p60, LUX Wermelskirchen, Germany). View Full Text PDF Listings View primary source full text article PDFs. The genes were predicted to encode a helicase, an intercapsomeric triplex protein, the DNA polymerase and the major capsid protein. The complete genomes of three CyHV-3 isolates showed more than 99% of DNA sequence identity, with the majority of differences located in short tandem repeats, also called VNTR (variable number of tandem repeats). Sequence accession numbers [ ] and assigned abbreviations ( ) are also listed.