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In this study, a total of 790 NPEVs isolated from stool samples submitted to the National Reference Laboratory from 1992 to 2008 were analyzed; neutralization test was able to type 55% (442) of the isolates. Enteroviruses are now increasingly being detected by PCR rather than by culture. Scopus) due to meningitis and meningoencephalitis from January 1993 to January 2014 were included in this retrospective study. Direct IF of vesicular cell smears EM of vesicle cell smears – cannot be distinguished from VZV particles. Isolation of virus RNA by a silica-based RNA extraction method was compared with the nonmagnetic and magnetic NucliSens RNA isolation methods. These results suggest that the combination of both virus isolation from environmental sources and phylogenetic analysis could be complementary assessment approaches to trace prevalent and minor circulating enteroviruses in the human population. Peaks of E30 isolations occurred in the years 1968 to 1969, 1981 to 1984, 1990 to 1993, and 1997 to 1998, coincident with large nationwide outbreaks of E30-associated aseptic meningitis.

An outbreak of echovirus 33 in schools in China in 2013. We prospectively recruited 749 healthy neonates and conducted follow-ups from June 2006 to December 2009. Of 42 enterovirus-positive samples, NASBA detected 39 (92.9%) and RT-PCR detected 37 (88.1%). The year 2011 experienced the largest HFMD epidemic since the start of National Epidemiological Surveillance of Infectious Diseases (NESID) (July 1981). These isolates were identified as PV by neutralization test, and 42 strains were identified as single-type PV (PV1 = 8, PV2 = 23, PV3 = 11) and 9 strains were identified as mixed-type PV [PV(1 + 2) = 5, PV(1 + 3) = 2, PV(2 + 3) = 2]. This review describes advances in cell culture-based viral diagnostic products and techniques, including the use of newer cell culture formats, cryopreserved cell cultures, centrifugation-enhanced inoculation, precytopathogenic effect detection, cocultivated cell cultures, and transgenic cell lines. The viruses were isolated in green monkey kidney cell cultures, in newborn cotton rats, newborn white mice and in monkeys.


Conventional culture detected 74 enterovirus isolates. Oberste, M., Schnurr, D., Maher, K., al-Busaidy, S. We prospectively studied children with suspected HEV-71 (i.e., hand-foot-and-mouth disease, CNS disease, or both) over 3.5 years, using detailed virological investigation and genogroup analysis of all isolates. To increase yield and enhance the rapidity of enterovirus isolation in cell cultures, we used Buffalo green monkey kidney (BGM) cells and subpassages of primary human embryonic kidney (HEK) cells in addition to the human diploid fibroblast (MRC-5) cells and primary cynomolgus or rhesus monkey kidney (MK) cells routinely used for enterovirus culturing. A series of unrelated viral isolates as well as CSF samples from patients with meningitis/encephalitis or neurological syndromes unrelated to enterovirus infection were included as controls. A series of unrelated viral isolates as well as CSF samples from patients with meningitis/encephalitis or neurological syndromes unrelated to enterovirus infection were included as controls. This method of determining infectious titres is recommended for improving enterovirus isolation in other epidemics.

High viral titre in clinical specimens resulted in rate increase for isolation in Caco-2 cells and RD cells (87.5% and 50%, respectively). Specificity of IgM EIA and of complement fixation was 94% and 85%, respectively. Enteroviruses (echovirus 6, 21 and 30) were isolated from cerebrospinal fluid (CSF) in 26/37 (70%) and from stool samples of 20/21 (95%) of patients with no other etiology. What is known about the pathogenesis of the infection? Viral nucleic acid was detected in 138 (8.2%) of the CSF samples, including enteroviruses in 51 samples, HSV-2 in 33 samples, VZV in 28 samples, and HSV-1 in 25 samples. Differing provisions from the publisher’s actual policy or licence agreement may be applicable. The prevailing enterovirus serotypes change from year to year as well as from area to area.

Background: Detection of enteroviral nucleic acid in cerebrospinal fluid (CSF) specimens has been demonstrated to improve the management of patients with aseptic meningitis. Correct virus isolation results were obtained for 105 of 110 samples (95.5%, four false-negatives, one false-positive), and correct PCR results for 39 of 40 (97.5%, one false-negative). Enteroviruses (echovirus 6, 21 and 30) were isolated from cerebrospinal fluid (CSF) in 26/37 (70%) and from stool samples of 20/21 (95%) of patients with no other etiology. Samples were referred from 1,013 patients; virus isolation was attempted from 579 CSF specimens and from 400 stool samples. Symptoms associated with aseptic meningitis infection in patients included the occurrence of headaches and mild fever. 2016 Jan. EM examined cerebrospinal fluid of all patients and virus isolation in tissue cultures was performed in all cerebrospinal fluid samples.

The potential for blood, hemoglobin, white blood cells, and excess protein to interfere with the assay was also assessed. Peaks of E30 isolations occurred in the years 1968 to 1969, 1981 to 1984, 1990 to 1993, and 1997 to 1998, coincident with large nationwide outbreaks of E30-associated aseptic meningitis.