All herpes viruses currently known (human and non-human) encode multiple miRNAs . In a particular aspect, there is provided herein a method where the miR profile involves at least one miRNA selected from the group consisting of miR-200a, miR-200b, miR-200c and miR-141, wherein a difference in expression of one or more of the miRNA compared to a normal sample is indicative of serous ovarian cancer. Investigators have modified oHSV to incorporate various constructs in order to enhance their cytotoxic and immune-stimulating effects. When injected directly into the abdominal cavities of mice bearing human ovarian cancer xenografts, Synco-2D induced complete remissions in 75% of the treated animals, in contrast to partial remissions in the group treated with a conventional oncolytic HSV. Luminescence images were taken the day after the HPV-16/Luc psV injection. The two cis-acting sequences from HSV-1, namely “pac,” in which the HSV packaging signal is located, and “oris,” the HSV-1 origin of DNA replication, are required for amplicon genome amplification and packaging. For example, the combination of Ad5/3-Δ24 with gemcitabine (a nucleoside analog), or epirubicin (a DNA-intercalating anthracycline drug) resulted in greater therapeutic efficacy than either agent alone in ovarian cancer xenograft models.110 Combination of dl922–947 with paclitaxel or combination of the survivin promoter–containing vector CRAd.S-RGD with cisplatin also had significantly greater therapeutic benefits than single-agent treatments in ovarian cancer xenograft models.111,112 It was suggested that cisplatin and gemcitabine might act to increase viral replication, whereas paclitaxel and dl922–947 together induced aberrant mitotic slippage and multinucleation, leading to a more efficient apoptotic cell death.
This protocol is novel in the site of administration of the HStk VPC and the dose escalation schema. This study reports that the combination of HSV1790 and the prodrug CB1954 enhanced tumor cell killing in vitro and increased tumor reduction and improved survival in vivo compared to the oncolytic effects of HSV1790 alone. OVCAR-3, SKOV3 and SKOV3-ip1-fLuc were maintained in α-Minimal Essential Medium (αMEM) supplemented with 20% FBS and penicillin/streptomycin (100 U/mL and 100 μg/mL) at 37°C and 5% CO2. It is conceived that arming the engineered T cells with such a capability would greatly improve their therapeutic outcome. Two thirds of women present with progressive disease wherein the cancer has already disseminated to abdominal organs or distant sites (1). Chen, Y., Chen, H., Rhoad, A.E. DNA laddering after virus infection.
Andrew Wakefield; this diagnostic terminology, however, has been questioned by medical experts. I will leave it to my oncologist – more experienced than I am. Clearly, the availability of viruses with selective tropism to the tumor cells would allow the use of less attenuated viruses with higher oncolytic activity. The cells were infected with HPV-16/Luc psV (1 µg L1 protein/ml) for 72 hours. In the natural life cycle of HSV-1, a critical step for the successful infection of any cell by a viral particle is its initial attachment and entry into the cell. The most popular and widely used TAAs for tumor vaccines are HLA-restricted immunodominant peptides. Crit Rev Oncol Hematol 60: 159-179.
The cell, not the virus, determines the choice as to which pathway of entry is taken by HSV. One cause of hyperbilirubinemia in seemingly healthy full-term or near-term infants is biliary atresia , an obstruction or inflammation of the bile ducts. Donovan Cancer Research Program and by a liberal donation from Dr. Trichostatin A (TSA) belongs to a group of compounds termed histone deacetylase (HDAC) inhibitors (HDACIs). 2. These results show that Fuson-H2 is an effective oncolytic agent for treatment of metastatic human ovarian cancer in the abdominal cavity 57. Results: T-01 and T-mfIL12 has great cytotoxicity for human and mouse cervical cancer cell lines.
The oncolytic herpes simplex virus 34.5ENVE is engineered to target cancer cells that overexpress the protein nestin and to inhibit the growth of blood vessels to tumors. The survival of mice treated with a high-titer hrR3 (5 x 10(7) plaque-forming units [PFU]) was significantly prolonged as compared with the group given paclitaxel (P < 0.0001, log-rank test). HSV-G207 was shown to penetrate deeply within tumor nodules and caused no apparent intraperitoneal toxicity. When the multiplicities of infection (MOI) was 100, the transduction rate was 98.9%. The tumor inhibition rates in 3AO/VP22 group and 3AO/VP22-TK group were 37.7% and 91.5% respectively after administration of 10 mg/kg GCV (P < 0.01), and were 81.8% and 96.7% respectively after administration of 50 mg/kg GCV (P > 0.05). Construction of an oncolytic herpes simplex virus that precisely targets hepatocellular carcinoma cells. MiRNAs sequencing data from 487 SEOC patients were downloaded from the TCGA website and analyzed through in-house sequencing pipeline.
They used data from the UK Million Women Study – a longitudinal study of 1,146,985 women aged over 50 at recruitment. We found that the one time and continuous exogenous gene transfer efficiencies were about 80% and 85%, respectively. The growth inhibitory rate of AO cell line transfered by pLNS/HSV-TK was 98.0% in the medium containing 400 mumol GVC.