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The cornea graft was secured with eight interrupted 11-0 nylon sutures (Sharpoint, Nylon black monofilament, 5″/13cm, 0.1 metric, 11-0; Surgical Specialties Corporation, Reading, PA, USA). The cornea graft was secured with eight interrupted 11-0 nylon sutures (Sharpoint, Nylon black monofilament, 5″/13cm, 0.1 metric, 11-0; Surgical Specialties Corporation, Reading, PA, USA). All image reconstructions were made using MetaMorph (version: 7.5.4.0; Molecular Devices, Sunnyvale, CA, USA). In November 2009, the patient presented in our clinic with a persisting epithelial defect OS, which did not heal despite intensive topical therapy with autologous serum (Figure 1C). Despite treatment with a bandage contact lens and topical antibiotics the epithelial defect persisted with eventual extension of the epithelial defect onto the host cornea. Twenty transformants were subjected to PCR analysis with virus specific primers. The EBAA reviews all reported adverse events associated with corneal transplantation and regularly assesses the need for changes in eye banking standards.

Graft resuturing for traumatic graft dehiscence was done for two patients. Anterior chamber examination showed 1+ cells, and no Khodadoust line was observed. The study mainly focused on nonhealing sterile corneal ulcers, and all patients received corneal scraping, culture, and laser scanning confocal microscopy examination to exclude active infection, for example, a fungal, resistant bacterial, or Acanthamoeba corneal ulcer. More information can be found at blepharitis medical treatment and blepharitis surgical treatment. Confocal microscopic analysis was performed on a Leica SP2 confocal system (Leica Microsystems, Bannockburn, IL) using sequential scanning to minimize bleed-through. The amniotic membrane (AM) was prepared and preserved as previously reported [7], with minor modifications. Four patients died at various stages of follow‐up, with a mean time to death of 44 months.


But as the dystrophy worsens, this swelling will remain constant and reduce vision for longer and eventually blurred vision remains throughout the day. For this reason, during the first year, an antiviral is administered orally as a prophylactic during the postoperative period. Corneas were excised, washed in PBS with 4% fetal bovine serum (FBS) for 10 minutes at 4°C prior to fixation in CytoFix/CytoPerm24 for 1 hour at 4°C. Donor corneas were stored in tissue culture medium (Optisol GS) for up to 7 days or organ culture medium for up to 28 days before surgery. The surgery was improved so that penetrating keratoplasty began to be performed on a more regular basis during the 1930s and 1940s in various countries. Corneal ulcer — Corneal ulcers may be sterile or caused by a bacteria, virus, fungus or parasite. Extreme care is taken to avoid penetration into the anterior chamber, since one of the advantages of LKP is that it is not necessary to enter the eyeball.

I usually have the patient present for follow-up within three days after membrane placement. In general, this means that patients with unilateral disease and perfect vision in the other eye are poor candidates. Patients diagnosed with herpetic infections that led to keratoplasty, pregnant women, and patients who refused to sign the informed consent form were excluded from the study. Standard keratomileusis (ALK) is considered investigational for treatment of all refractive errors because its effectiveness for trestment of refractive errors has not been proven. This study was approved by the University of Texas Southwestern (UTSW) Medical Center Institutional Review Board (IRB). Larger grafts appear to increase the risk of graft rejection. The data obtained was analysed statistically by the Mann–Whitney U-test (one tailed) to ascertain if patients operated on by a corneal surgeon had better outcomes.

Avoid wearing contact lenses overnight. The causes of active infectious corneal ulcers were determined by clinical findings (especially for active herpes simplex keratitis), confocal biomicroscopy (Confoscan 3.4; Nidek Co. Visual acuity was 20/200 OD. However, the intervention in human HSV-1 keratitis patients using exogenous IFN-α has been controversial with reports of efficacious and non-efficacious results (Sundmacher et al., 1976; Coster et al., 1977; Minkovitz et al., 1995). These cells do not regenerate but instead stretch to compensate for dead cells. He has published widely in peer-reviewed journals including Ophthalmology, JAMA Ophthalmology and the British Journal of Ophthalmology. A number of risk factors for graft failure were examined: in most instances, there was little room for decision-making or expert intervention.

Associated anterior segment findings included iris prolapse in 71.9%, lens extrusion in 34.4%, and hyphema in 40.6% of eyes. The principal functions of the cornea in vision are to permit transmission of light into the eye and to focus it onto the retina. Immunohistochemistry and DNA-polymerase chain reaction were negative for herpes simplex virus-1 (HSV-1) in the host cornea, but they detected HSV-1 in both transplanted corneal grafts, thereby supporting our clinical hypothesis that graft-to-host HSV-1 infection elicited this chain reaction of complications in our patient. Immune rejections remain one of the most common causes of failure of penetrating keratoplasty. To report the indications for and techniques of corneal transplantation at Vietnam National Institute of Ophthalmology (VNIO) over a period of 12y (2002-2013).